Synaptopodin (SYNPO) (N-term) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting (Western): 1/500-1/1000. Immunofluorescence. Immunohistochemistry on Frozen Sections: 1/50-1/100. Immunohistochemistry on Paraffin Sections: 1/50-1/100 (after microwave treatment). Incubation Time:1 h at RT for immunohistochemistry.
Reactivity	Human, Mouse
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptides (Mouse N-terminus), coupled to KLH
Specificity	The antibody reacts specifically with Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the cor-responding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to post-translational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.  The antibody recognizes differentiated podocytes (glomerular visceral epi-thelial cells) in vivo and in vitro (weaker additional reaction with arterial endo-thelial cells), co-localization with alpha-actinin. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentia-tion-dependent expression in cultured hippocampal neurons.
Formulation	State: Serum State: Liquid Stabilized Antiserum Preservative: 0.09% Sodium Azide Stabilizer: 0.5 % BSA
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Gene Name	synaptopodin
Database Link	Entrez Gene 11346 Human UniProt ID Q8N3V7
Synonyms	KIAA1029
Reference Data

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