PDGF Receptor alpha (PDGFRA) Mouse Monoclonal Antibody [Clone ID: 57/20 (35248)]

Specifications

Product Data
Clone Name	57/20 (35248)
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 0.5-1.0 µg/ml. Western Blot: 1-2 µg/ml. Immunohistochemistry on Paraffin Embedded Sections: 10-20 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.
Specificity	This antibody detects PDGF RECEPTOR ALPHA. The ND50 was determined as 0.2-0.6 µg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of Human WS1 cells.
Isotype	IgG1
Formulation	PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and 10% Trehalose as stabilizer. State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet derived growth factor receptor alpha
Database Link	Entrez Gene 5156 Human UniProt ID P16234
Background	PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cellsurface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases.
Synonyms	PDGF-R-alpha, PDGF Receptor alpha
Reference Data

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