Complement C5 (C5) (neoepitope) Mouse Monoclonal Antibody [Clone ID: HCC5b.1 (neo)]

Specifications

Product Data
Clone Name	HCC5b.1 (neo)
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Reactivity	Bovine, Goat, Human, Porcine, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Activated Human Complement Component C5
Specificity	HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000; after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.
Isotype	IgG1
Formulation	PBS State: Purified State: Lyophilized purified Ig fraction from Ascites. Stabilizer: 0.5% BSA Preservative: 0.09 % sodium azide
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	complement component 5
Database Link	Entrez Gene 727 Human UniProt ID P01031
Background	C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Synonyms	Complement Component 5, CPAMD4
Note	Antibody HCC 5.1, cat.no. BM5030 reacts with native C5.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus

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