Corticotropin Releasing Factor (CRH) Mouse Monoclonal Antibody [Clone ID: 4H9]

Specifications

Product Data
Clone Name	4H9
Applications	ELISA, IHC
Recommend Dilution	Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin sections: 1/100 - 1/7000; Microwave pretreatment is required. Enzyme digestion with 0.1% trypsin may also be necessary. ELISA: This antibody is suitable for use in a sandwich ELISA with clone 5D5 (BM356).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.
Specificity	This antibody is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland. The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
Isotype	IgG1
Formulation	PBS containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	corticotropin releasing hormone
Database Link	Entrez Gene 1392 Human UniProt ID P06850
Background	CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
Synonyms	CRF, CRH, Corticotropin-Releasing Hormone, Corticotropin-Releasing Factor
Reference Data

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