Macrophages (pan) Mouse Monoclonal Antibody [Clone ID: RM1]

Specifications

Product Data
Clone Name	RM1
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 2 µg/ml (1/100). Immunohistochemistry on Paraffin Sections: 20 µg/ml (1/10). Microwave pretreatment for antigen retrieval is recommended. Has been described to work in FACS. Suggested Positive Control: Rat spleen.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Peritoneal Macrophages.
Specificity	This antibody detects monocytes, subpopulation of macrophages, interdigitating cells. The antigen is localized on the surface of cells. It is stable to 2% periodate-lysine-paraformaldehyde fixation. The epitope has not been further characterized. Antigen Distribution: Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % of peritoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
Isotype	IgG1
Formulation	PBS pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as a preservative. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
Synonyms	Macrophage marker
Reference Data

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