B-Cells (pan) Mouse Monoclonal Antibody [Clone ID: Ki-B1R]

Specifications

Product Data
Clone Name	Ki-B1R
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen sections: 1 µg/ml (1/100). Immunohistochemistry on Paraffin sections: 10 µg/ml (1/10). No antigen retrieval required, microwave treatment may enhance the reactivity. Suggested Positive Control: Rat spleen. Has been described to work in FACS.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat lymphocytes
Specificity	Monoclonal Rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections. Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The antibody is specific for Rat mature B-lymphocytes and plasma cells. Other species not tested. Antigen Distribution: Isolated Cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% +/- 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue Sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo.
Isotype	IgG1
Formulation	PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative State: Purified State: Lyohilized purified IgG fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.1 mg/ml (after reconstitution)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.
Synonyms	B-cell marker, B cells
Reference Data

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