Macrophages (Haematopoiesis associated) Rat Monoclonal Antibody [Clone ID: ER-HR3]

Specifications

Product Data
Clone Name	ER-HR3
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Flow Cytometry. The typical starting working dilution for the applications is 1/50.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	The monoclonal antibody ER-HR3 is reactive to the majority of Mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few ER-HR3 positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen. The monoclonal antibody ER-HR3 can be used to identify and localize a very distinct mature tissue macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
Isotype	IgG2c
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Synonyms	Macrophage marker
Reference Data

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