Macrophages Mouse Monoclonal Antibody [Clone ID: PM-2K]

Specifications

Product Data
Clone Name	PM-2K
Applications	IHC
Recommend Dilution	Immunohistochemistry on frozen section: 0.2 mg/ml (1:1000). Suggested positive control: Human tonsil. Does not react on routinely processed paraffin sections.
Reactivity	Bovine, Canine, Feline, Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Cultured human peritoneal macrophages
Specificity	This antibody detects Macrophages. Antigen distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes. Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages an d macrophages in the interstitial tissues of the kidney, pancreas and many other organs. Proliferating macrophages are positive i n Gaucher's disease , multicentric reticulohistiocytosis and malignant histiocytosis. In MFH infiltrating macrophages are staine d but not tumour cells. Some bone stromal cells but not osteoclast - like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are nega tive.
Isotype	IgG1
Formulation	Phosphate buffered saline pH 7.2 (PBS) State: Purified State: Lyophilized affinity purified Ig fraction Stabilizer: 5 mg/ml bovine serum albumin (BSA) Preservative: 0.05 % (v/v) Kathon CG
Reconstitution Method	Reconstitute by adding 0.5 ml distilled water.
Purification
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Monoclonal antibody PM-2K, together with X-4 and X-14, forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop. The PM-2K antigen was initially suspected to function as a scavenger receptor. However, experiments with CHO cells expressing Macrophage Scavenger Receptor (MSR) AI or AII showed no reactivity with this antibody.
Synonyms	Macrophage marker
Reference Data

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