p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: BP53-12]

Specifications

Product Data
Clone Name	BP53-12
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western Blotting (Non-reducing conditions): Recommended Dilution: 1-2 µg/ml, overnight in 4°C. Positive Control: RAMOS Human lymphoma cell line Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. SDS-PAGE: 12% separating gel. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially expressed full-length wild-type p53.
Specificity	This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
Isotype	IgG2a
Formulation	Phosphate buffered saline (PBS), pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Precipitation Methods
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Background	The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

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