MHC Class I H-2 (b,d,q,h4,m,w16) Rat Monoclonal Antibody [Clone ID: ER-HR52]

Specifications

Product Data
Clone Name	ER-HR52
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1 µg/ml (1/200). Recommended Positive Control: Mouse Spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse macrophage precursor cells
Specificity	ER-HR52 is useful for detecting MHC class I antigens. It is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells. The antigen is expressed by all somatic cells at varying levels. ER-HR52 detects MHC class I antigens of various haplotypes (see below). The antigen is found on all somatic cells in all organs sections though at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression.
Isotype	IgG2a
Formulation	PBS, pH 7.2 containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore by adding 0.5 ml distilled water.
Concentration	0.2 mg/ml
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the original vial at 2-8°C and the reconstituted stock solution (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Note	Protocol: Protocol with Frozen, ice-cold Acetone-Fixed Sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L) minimal-cross reaction to mouse) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum.
Reference Data

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