MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

Specifications

Product Data
Clone Name	ER-TR3
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 4 µg/ml (1/100). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Murine thymic reticulum
Specificity	This antibody detects cells expressing MHC class II antigens. Monoclonal antibody ER-TR3 detects MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. it is a valuable tool for studying T-helper cell interaction with class II positive antigen presenting cells (dendritic cells, B-cells, macrophages). This antibody also offers new possibilities for studying the development of T-helper cells since it also stains stromal cells in the thymus. Antigen Distribution Isolated Cells: The antigen is found on dendritic cells, B-cells and macrophages. Tissue sections: The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
Isotype	IgG2b
Formulation	Stock Solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer with no preservative Label: Biotin State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C and reconstituted at -20°C. Avoid repeated freezing and thawing.
Background	MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.
Reference Data

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