T Cells (Pan) Mouse Monoclonal Antibody [Clone ID: KI-T1R]

Specifications

Product Data
Clone Name	KI-T1R
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.25 μg/ml (1/800). Ki-T1R shows very charateristic staining patterns in lymphoid organs according to the T-cell distribution; paracortical T-cell zone of lymphnodes, periarteriolar T-cell zones of the spleen, T-cell nodules of the gastrointestinal tract and bone marrow, thymocytes with prevalence from thymus medulla. This antibody has been described to work in FACS.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat lymphocytes.
Specificity	This antibody reacts with Rat B-lymphocytes. This Monoclonal antibody Ki-T1R is a pan-T-cell marker useful for the characterisation of lymphoid organs and for the study of the interaction of T-cells with other cell types e.g. Macrophages. Ki-T1R detects an epitope of a 161kD glycoprotein (4 bands of 36kD, 38kD, 40kD and 47kD under reducing conditions) which is located on the outer surface of cytoplasmic membranes. Ki-T1R detects approximately 80% of peripheral blood T-cells. It is negative on all other cell types.
Isotype	IgG2a
Formulation	PBS, pH 7.2 with 0.01% Thimerosal as preservatives and 10 mg/ml BSA as stabilizer. Label: Biotin State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Monoclonal antibody Ki-T1R is a pan-T-cell marker useful for the characterisation of lymphoid organs and for the study of the interaction of T-cells with other cell types e.g. macrophages.
Synonyms	T-Cells, T Lymphocytes, T-lymphocytes
Reference Data

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