Desmocollin 1 (DSC1) Mouse Monoclonal Antibody [Clone ID: Dsc-1-U100]

Specifications

Product Data
Clone Name	Dsc-1-U100
Applications	IHC, WB
Recommend Dilution	Imunoblotting (Western). Immunohistochemistry on Frozen Sections: For better resolution on Frozen Sections preincubation (directely after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type is recommended (see Ref.2). Without detergent pretreatment a cytoplasmic component might cross-react (e.g. in fibroblasts). Immunohistochemistry on Paraffin-Embedded Sections (after microwave treatment): A slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step. Working Dilution: After reconstitution dilute further 1/10 in PBS (pH 7.4) for Immunohistochemistry. Incubation Time: 1h at RT for Immunohistochemistry or overnight at 2-8°C.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1
Specificity	Reacts with 111 kD (form "a") and 103 kD (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes. Mab localizes Dsc1 present in - suprabasal layers of interfollicular epidermis - specific cell layers (e.g. in hair follicle root sheath) - Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).
Isotype	IgG1
Formulation	PBS buffer, pH 7.4 containing 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 1 ml dist. water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Stable for one year after reconstitution when stored at 2-8°C. For extended storage keep in aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Gene Name	desmocollin 1
Database Link	Entrez Gene 1823 Human UniProt ID Q08554
Background	Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.
Synonyms	DG2/DG3, DSC1, CDHF1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.