Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: Ks18.27]

Specifications

Product Data
Clone Name	Ks18.27
Applications	ELISA, IHC, WB
Recommend Dilution	Immunohistochemistry on frozen sections: 1:10; 1 h at RT. Cytological material. Immunoblotting. ELISA.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal preparation from tumor cell line MCF-7.
Specificity	Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18; formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine; negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA.
Isotype	IgG1
Formulation	PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5% BSA State: Purified State: Lyophilized purified IgG
Reconstitution Method	Restore in 1 ml dist. water
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 3875 Human UniProt ID P05783
Background	Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data

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