Nucleophosmin (NPM1) Mouse Monoclonal Antibody [Clone ID: No-63]

Specifications

Product Data
Clone Name	No-63
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunoblotting: 1:1000 - 1:2000; ECL method; for blotting transfer nitrocellulose sheets are superior to nylon sheets. Immunoprecipitation: from Xenopus oocyte extracts nucleoplasmin - a 30 kD protein - is co-precipitated, which shows high sequence homology to NO38. Immunofluorescence microscopy. Immunohistochemistry on frozen sections: 1:300 - 1:500; 1 h at RT; also after fixation with paraformaldehyde.
Reactivity	Chicken, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	Nucleolar fraction prepared from Xenopus laevis oocytes
Specificity	The antibody recognizes a 38 kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
Isotype	IgG2a
Formulation	State: Ascites State: Liquid Ascites containing 0.09% NaN3
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in small aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Database Link	UniProt ID P06748
Synonyms	NPM, NPM1, Numatrin
Reference Data

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