Borrelia burgdorferi Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections. Acetone fixation of the antigen source is recommended prior to staining. Direct IFA staining of target antigens in a permissive tissue culture system. Western blot.
Reactivity	Borrelia burgdorferi
Host	Rabbit
Clonality	Polyclonal
Immunogen	Whole cell preparation from B. burgdorferi.
Specificity	Reacts with 83, 41, 34 and 31 kD proteins and their fragments in Western blots. Reacts also with T. pallidum, B. hermsii and B. parkerii.
Formulation	0.01M PBS, pH 7.2 with 10 mg/ml BSA as stabilizer and 0.09% sodium azide as preservative Label: FITC State: Liquid purified Ig fraction. Label: Highly purified Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Background	B. burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
Reference Data

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