Collagen type I Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	ELISA. RIA: 1/1000. Immunfluorescence: 1/40-1/80. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500-1/1000. Incubation time: 60 min at RT or 2-8°C overnight. Positive Control: Chicken skin.  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity	Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified collagen type I from chicken skin
Specificity	Chicken Collagen type I: 100%.  Chicken Collagen type III and IV: < 0.1 %.  Collagen type I Human, Mouse, Rat, Cattle: < 0.1 %.  Chicken Fibronectin: < 0.1 % RIA at 1/1000 dilution.
Formulation	State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore by adding 0.1 ml aqua destilled, for further dilution use appropriate antibody diluent.
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P02457
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.