Sonic Hedgehog (SHH) Mouse Monoclonal Antibody [Clone ID: OTI10H6]

CAT#: CF500041

Carrier-free (BSA/glycerol-free) SHH mouse monoclonal antibody, clone OTI10H6 (formerly 10H6)

Formulation: Standard Carrier-Free



  View other "OTI10H6" antibodies (4)

Need it in bulk or conjugated?
Get a free quote

CNY 3,999.00


货期*
2周

规格
    • 100 ug

Product images

经常一起买 (2)
Biotinylation Labelling Kit formatted 4 x 0.05mg
    • 200 ug

CNY 3,170.00


HRP Conjugation kit for 0.2mg IgG
    • 200 ug

CNY 3,230.00

Specifications

Product Data
Clone Name OTI10H6
Applications IF, WB
Recommend Dilution WB 1:1000~2000, IF 1:100
Reactivity Human, Dog, Rat, Monkey, Mouse
Host Mouse
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 24-197 of human SHH (NP_000184) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 27.6 kDa
Gene Name sonic hedgehog signaling molecule
Background SHH is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.
Synonyms HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...