TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]

Specifications

Product Data
Clone Name	OTI8D11
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:1000~2000, IHC 1:50, IF 1:50, FLOW 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TRIB2 (NP_067675) produced in HEK293T cell.
Isotype	IgG2b
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.8 kDa
Gene Name	tribbles pseudokinase 2
Database Link	NP_067675 Entrez Gene 217410 MouseEntrez Gene 313974 RatEntrez Gene 28951 Human UniProt ID Q92519
Background	This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.
Synonyms	C5FW; GS3955; TRB2
Note	WB application: Only overexpression results are guaranteed, not endogenous.
Reference Data
Protein Families	Druggable Genome, Protein Kinase

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