XRCC4 Mouse Monoclonal Antibody [Clone ID: OTI4H9]

Specifications

Product Data
Clone Name	OTI4H9
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:50
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38.3 kDa
Gene Name	X-ray repair cross complementing 4
Database Link	NP_072044 Entrez Gene 7518 Human UniProt ID Q13426
Background	The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants.
Synonyms	SSMED
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Non-homologous end-joining

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