Lipoamide Dehydrogenase (DLD) Mouse Monoclonal Antibody [Clone ID: OTI8A10]

Specifications

Product Data
Clone Name	OTI8A10
Applications	FC, WB
Recommend Dilution	WB 1:500, FLOW 1:100
Reactivity	Human, Dog, Rat, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	50.1 kDa
Gene Name	dihydrolipoamide dehydrogenase
Database Link	NP_000099 Entrez Gene 13382 MouseEntrez Gene 298942 RatEntrez Gene 403978 DogEntrez Gene 700494 MonkeyEntrez Gene 1738 Human UniProt ID P09622
Background	This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq, Jul 2008]
Synonyms	DLDD; DLDH; E3; GCSL; LAD; PHE3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation

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