BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5]

Specifications

Product Data
Clone Name	OTI3F5
Applications	IHC, Simple Western, WB
Recommend Dilution	WB 1:500~2000, IHC 1:150, Simple Western 1:20-1:50
Reactivity	Human, Dog, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.
Isotype	IgG2a
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	42.8 kDa
Gene Name	branched chain amino acid transaminase 1
Database Link	NP_005495 Entrez Gene 12035 MouseEntrez Gene 486633 DogEntrez Gene 586 Human UniProt ID P54687
Background	This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq]
Synonyms	BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation

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