Mannose Phosphate Isomerase (MPI) Mouse Monoclonal Antibody [Clone ID: OTI1C7]

Specifications

Product Data
Clone Name	OTI1C7
Applications	FC, IF, IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human MPI(NP_002426) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46.5 kDa
Gene Name	mannose phosphate isomerase
Database Link	NP_002426 Entrez Gene 300741 RatEntrez Gene 4351 Human UniProt ID P34949
Background	Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq]
Synonyms	CDG1B; PMI; PMI1
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways

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