KAP1 (TRIM28) Mouse Monoclonal Antibody [Clone ID: OTI8B12]

Specifications

Product Data
Clone Name	OTI8B12
Applications	IF, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:500, IF 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human TRIM28 (NP_005753) produced in HEK293T cell.
Isotype	IgG1
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	88.4 kDa
Gene Name	tripartite motif containing 28
Database Link	NP_005753 Entrez Gene 21849 MouseEntrez Gene 116698 RatEntrez Gene 10155 Human UniProt ID Q13263
Background	The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Synonyms	KAP1; PPP1R157; RNF96; TF1B; TIF1B
Reference Data
Protein Families	Protein Kinase, Stem cell - Pluripotency, Transcription Factors

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