MHC Class I (RT1Ac) Mouse Monoclonal Antibody [Clone ID: OX-27]

Specifications

Product Data
Clone Name	OX-27
Applications	FC
Recommend Dilution	Flow cytometry: use 10 µl of neat antibody to label 10e6 cells in 100 µl.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	PHA activated rat lymphocytes
Specificity	This antibody recognises a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Isotype	IgG2a
Formulation	PBS containing 1% BSA and 0.09% sodium azide Label: PE State: Lyophilized purified IgG fraction Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Protein G chromatography
Conjugation	PE
Storage Condition	Prior to and following reconstitution store (undiluted) at 2-8°C. DO NOT FREEZE. This product is photosensitive and should be protected from light.
Database Link	UniProt ID Q31255
Background	MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
Synonyms	RT1-A3
Reference Data

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