Lamp3 Rat Monoclonal Antibody [Clone ID: 1006F7.05]

Specifications

Product Data
Clone Name	1006F7.05
Applications	FC
Recommend Dilution	DDX0192P-50 / DDX0192P-100 Purified: Surface and Intra Flow cytometry, IHC, IF. DDX0192A488-50 / DDX0192A488-100 Alexa-fluor®488: Surface and Intra Flow cytometry, IF. DDX0192A546-50 / DDX0192A546-100 Alexa- fluor®546: Surface and Intra Flow cytometry. DDX0192A647-50 / DDX0192A647-100 Alexa- fluor®647: Surface and Intra Flow cytometry. DDX0192B-50 / DDX0192B-100: Biotin (on request): Surface and Intra Flow cytometry, IHC. Usage recommendation: *This monoclonal antibody may be used between 5-20μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	recombinant murine DC-LAMP
Specificity	Murine DC-LAMP Species cross-reactivity: none.
Isotype	IgG2a
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol Label: Alexa Fluor 647
Concentration	lot specific
Purification	QMA hyper D ion exchange chromatography
Conjugation	Alexa Fluor 647
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	lysosomal-associated membrane protein 3
Database Link	Entrez Gene 239739 Mouse UniProt ID Q7TST5
Background	The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. DC-LAMP is specifically expressed by human DCs upon activation. However, its mouse counterpart has not be detected in mature DCs. DC-LAMP is constitutively expressed by mouse. Confocal and immunoelectron microscopy have shown that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contains lung surfactant protein B. (Salaun B et al, 2003 ; Eur. J. Immunol., 33 :9, 2619-29 ; Salaun B et al, 2004 ; Am. J. Pathol., 164 :3, 861-71 ; Rochereau N et al, Vaccine, 2011).
Synonyms	DCLAMP, DC-LAMP, TSC403, Dendritic Cell Marker
Reference Data

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