IRAK4 Mouse Monoclonal Antibody [Clone ID: 6F8]

Specifications

Product Data
Clone Name	6F8
Applications	FC, IF
Recommend Dilution	DDX0340P-50 / DDX0340P-100 Purified: IHC paraffin, IF, Intracellular flow cytometry, WB. DDX0340A488-50 / DDX0340A488-100 Alexa-fluor®488: IF, Intracellular flow cytometry. DDX0340A546-50 / DDX0340A546-100 Alexa-fluor®546: IF, Intracellular flow cytometry. DDX0340A647-50 / DDX0340A647-100 Alexa-fluor®647: IF, Intracellular flow cytometry. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use.
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	human recombinant IRAK-4 transfected 293T cells.
Specificity	Human IRAK-4. Species cross-reactivity: Dog.
Isotype	IgG1
Formulation	Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 546
Concentration	lot specific
Conjugation	Alexa Fluor 546
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Gene Name	interleukin 1 receptor associated kinase 4
Database Link	Entrez Gene 51135 Human UniProt ID Q9NWZ3
Background	IL-1R associated kinases (IRAKs) are important mediators in the signal transduction of Toll / IL-1R (TIR) family members. The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK-M and IRAK4). IL-1 stimulation leads to the recruitment of the IL-1R associated kinase (IRAK) to the IL-1 Receptor where IRAK is phosphorylated, ubiquinated, and eventually degraded. Endogenous IRAK-4 interacts with IRAK-1 and TRAF6 in an IL-1 dependent manner. IRAK-4 is able to phosphorylate IRAK-1. The 6F8 clone was generated after mouse immunization with human IRAK4-transfected eukaryotic cells. This monoclonal antibody was demonstrated to cross-react with canine IRAK-4. (Cao S. et al, 1996 ; Science, 271, 1128-1131 ; Li S. et al, 2002 ; PNAS, 99, 5567-5572, Bonnefont C, personal communication, 2011).
Synonyms	IRAK-4, NY-REN-64
Reference Data

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