MIP3 alpha / CCL20 Mouse Monoclonal Antibody [Clone ID: 319F6.07]

Specifications

Product Data
Clone Name	319F6.07
Applications	ELISA, FN, IHC
Recommend Dilution	DDX0430P-50 / DDX0430P-100 Purified: Blocking migration, ELISA capture, IHC
Reactivity	Human, Macaque
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant MIP3α in eukaryotic cells.
Specificity	Human MIP-3α. Species cross-reactivity: Macaque.
Isotype	IgG2b
Formulation	Purified: 100 μg in 200μl /50 μg in 100 μl Tris-NaCl pH 8 State: Purified
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	Macrophage inflammatory protein (MIP)-3α/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3α plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3α is the most potent chemokine inducing the selective migration of in vitro-generated CD34+ hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3α receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3α is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3α. (c) In vivo, MIP-3α is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3α upregulation appears associated with an increase in LC turnover. (d) The secretion of MIP-3α is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1β plus TNFα) or T cell signals. (Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18).
Synonyms	CCL20, LARC, MIP3A, MIP-3 alpha, C-C motif chemokine 20, Small-inducible cytokine A20, SCYA20, Exodus-1, Dendritic Cell Marker, DC marker
Reference Data

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