CD283 / TLR3 Mouse Monoclonal Antibody [Clone ID: 1205C5F1 / TLR3.1]

Specifications

Product Data
Clone Name	1205C5F1 / TLR3.1
Applications	FC, IHC, IP
Recommend Dilution	FACS intracellular, ImmunoHistoChemistry paraffin sections, Immunoprecipitation. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HEK-293 stably transfected with huTLR3+rhu TLR3 ECD (R&D)
Specificity	Human TLR3.
Isotype	IgG1
Formulation	Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol. Label: Alexa Fluor 647
Concentration	lot specific
Purification	QMA Hyper D ion exchange chromatography
Conjugation	Alexa Fluor 647
Storage Condition	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Background	The Toll-like receptor (TLR) family are considered as key for sensing microbial infections and triggering inflammatory and innate responses. 3 novel mAbs were generated against TLR3 that support the emergence of an alternative model for TLR3 biology: TLR3 full-length (TLR3FL, 130KDa) corresponds to the TLR3 present in ER, which in resting cells is steadily converted within the Golgi into fully glycosylated TLR3 (TLR3FL+, 135KDa) and then rapidly cleaved by cathepsins in the endosome/lysosome into 2 long-lived associated fragments (72 KDa and 60KDa). (Toscano F et al, J. Immunol, 2013, 190: 764–773).
Synonyms	Toll-like receptor 3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.