Glycophorin A (GYPA) Mouse Monoclonal Antibody [Clone ID: JC159]

Specifications

Product Data
Clone Name	JC159
Applications	FC, IHC
Recommend Dilution	Flow cytometry. Immunohistochemistry on paraffin sections: 10 µg/ml. Immunohistochemistry on frozen sections.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Membrane preparation from splenic hairy cell leukemia
Specificity	This antibody recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide, approx. pH 7.4
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95%pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	glycophorin A (MNS blood group)
Database Link	Entrez Gene 2993 Human UniProt ID P02724
Background	CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
Synonyms	Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein
Reference Data

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