CCR4 Mouse Monoclonal Antibody [Clone ID: KH-4F5]

Specifications

Product Data
Clone Name	KH-4F5
Applications	ELISA, FC, IF, WB
Recommend Dilution	Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400. Flow cytometry: 1.2 µg/106 cells. Immunofluorescence: 1 µg/106 cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Immunisation with Human CCR4 peptide.
Specificity	Recognizes Human CCR4. Other species not tested.
Isotype	IgG2a
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine receptor 4
Database Link	Entrez Gene 1233 Human UniProt ID P51679
Background	CCR4 (CC chemokine receptor 4) belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane-bound molecules composed of 7-transmembrane domains and are coupled to G-proteins (1,2). CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells (3,4). It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways (2,5).
Synonyms	C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CMKBR4, K5-5
Note	SDS-PAGE analysis: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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