MYOD1 Mouse Monoclonal Antibody [Clone ID: 5.2F]

Specifications

Product Data
Clone Name	5.2F
Applications	IHC, IP
Recommend Dilution	Immunoprecipitation: 1/50. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABC method for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1mM EDTA, pH 8.0 or 10mM Citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Rhabdomyosarcoma
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were injected with a recombinant mouse MyoD1 protein.
Specificity	This antibody is specific to a 45 kD protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas. Cellular Localization: Nuclear
Isotype	IgG2a
Formulation	State: Purified State: Liquid purified Ig fraction containing Sodium Azide as preservative.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	myogenic differentiation 1
Database Link	Entrez Gene 17927 Mouse UniProt ID P10085
Background	MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Synonyms	MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3, BHLHC1
Reference Data

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