FABP4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot. Immunohistochemistry on Frozen Sections.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunization antigen (14.7 kDa) is a protein containing 131 AA of recombinant Human FABP4 and one extra AA, N-terminal Methionin (highlighted).
Specificity	This antibody recognizes Adipocyte Fatty Acid Binding Protein (FABP4/AFABP).
Formulation	0.05 M Phosphate buffer, 0.1 M NaCl, pH 7.2 without preservatives. State: Azide Free State: Lyophilized purified IgG fraction.
Reconstitution Method	Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -20°C to -80°C. Reconstituted antibody can be stored at 2-8°C for a limited period of time; it does not show decline in activity after two weeks at 2-8°C. Avoid repeated freezing and thawing.
Gene Name	fatty acid binding protein 4
Database Link	Entrez Gene 2167 Human UniProt ID P15090
Background	Adipocyte fatty acid binding protein AFABP is a 15 kDa member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. AFABP is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells . In mice, targeted mutations in AFABP provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from AFABP-deficient mice also have reduced fficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated AFABP expression in macrophages upon differentiation and activation. In these cells, AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a central role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages.
Synonyms	A-FABP, Adipocyte lipid-binding protein, Fatty acid-binding protein 4
Reference Data

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