LYVE1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen and Paraffin Embedded Sections (1-5 µg/ml). Heat mediated antigen retrieval is recommended when staining paraffin embedded sections. Western blot (1-2 µg/ml). ELISA (1-15 µg/ml). Flow Cytometry (3-20 µg/ml).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Human soluble LYVE-1 produced in insect cells (Cat.-No DA3525). It consists of amino acid 24 (Ser) to 232 (Gly) and is fused to a C-terminal His-tag (6xHis).
Specificity	This antibody will detect LYVE-1 on the surface of lymphatic endothelial cells by Immunohistochemistry. It detects a 70 kD LYVE-1 band in Western blotting. This antibody is not reactive with Mouse LYVE-1.
Formulation	5mM PBS, pH 7.2 without preservatives or stabilizers State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Antigen Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	lymphatic vessel endothelial hyaluronan receptor 1
Database Link	Entrez Gene 10894 Human UniProt ID Q9Y5Y7
Background	LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosamino-glycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels.
Synonyms	LYVE1, CRSBP-1, CRSBP1, HAR, XLKD1
Reference Data

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