ansB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western blot: 1/500-1/3,000. Immunoprecipitation: 1/100. This product has been assayed against 1.0 µg of asparaginase [E. coli] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-rabbit IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Asparaginase from E. coli
Specificity	This antibody detects asparaginase [E. coli]. Cross reactivity against asparaginase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified asparaginase [E. coli].
Formulation	0.01 M Potassium phosphate, 0.14 M Sodium chloride, pH 7.4 State: Purified State: Lyophilized purified Ig fraction Stabilizer: None Preservative: 0.01% Sodium azide
Reconstitution Method	Restore with 0,1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00805
Synonyms	ansA, L-asparaginase I, L-ASNase I
Reference Data

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