Fructose 6 Phosphate Kinase Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	F6PK/PKF antibody has been tested for use in ELISA and Western blot. Recommended Dilutions: ELISA: 1/4,000-1/20,000. Western blot: 1/500-1/2,000. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK/PKF protein by western blotting in the appropriate cell lysate or extract.
Reactivity	Rabbit
Host	Goat
Clonality	Polyclonal
Immunogen	Full length native Fructose-6-phosphate kinase / Phosphofructokinase from rabbit muscle
Specificity	This antibody detects F6PK/PKF [rabbit muscle]. Cross reactivity against F6PK/PKF from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-goat serum as well as purified and partially purified F6PK/PKF [rabbit muscle].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00511
Background	Phosphofructokinase (PFK) catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Each subunit is encoded by a separate structural locus on chromosomes 1(M), 21(L), and 10(P). PFKL is the major form in liver and kidney while only M type PFK isozyme is expressed in mature muscle; therefore, muscle contains only homotetramers of M subunits. Erythrocytes contain both L and M subunits, and these randomly tetramerize to form M4, L4, and 3 additional hybrid forms of the holoenzyme.
Synonyms	6-phosphofructokinase muscle type, PFK1, PFKM, PFK-A
Reference Data

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