GST-Tag Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western: 1/1,000-1/5,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,500 to 1/8,000 of the reconstitution concentration is suggested for this product.
Reactivity	Schistosoma japonicum
Host	Goat
Clonality	Polyclonal
Immunogen	Glutathione-S-Transferase [Schistosoma japonicum].
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by Dlipidation and Defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P08515
Background	GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Synonyms	Glutathione S-transferase Tag, GST26-Tag
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.