Cyanine Dyes Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC
Recommend Dilution	Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended dilutions: This product has been assayed against 1.0 ug of Cyanine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Host	Sheep
Clonality	Polyclonal
Immunogen	A mixture of Cy3 and Cy5 conjugated KLH
Specificity	This product was prepared from monospecific antiserum by Immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% Sodium Azide as preservative and 10 mg/ml BSA as stabilzer, IgG and Protease free. Label: Biotin State: Lyophilized purified Ig fraction Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecule per sheep IgG molecule.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.