Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

Specifications

Product Data
Clone Name	NKI-GoH3
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Human Tonsil.
Reactivity	Canine, Human, Monkey, Mouse, Porcine, Sheep
Host	Rat
Clonality	Monoclonal
Immunogen	BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
Specificity	The monoclonal antibody is directed against the CDw49f-antigen. This monoclonal NKI-GoH3 antibody reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts. Platelet VLA-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by GoH3. Studies suggest that Clone NKI-GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (See Sonnenberg, A. et al. for details).
Isotype	IgG2a
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 6
Database Link	Entrez Gene 3655 Human UniProt ID P23229
Background	CDw49f-antigen is a 120kD, (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP 11a or VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.
Synonyms	Integrin alpha-6, VLA-6, VLA6
Reference Data

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