Thy1 Mouse Monoclonal Antibody [Clone ID: OX-7]

Specifications

Product Data
Clone Name	OX-7
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen and paraffin sections. Immunoprecipitation. Western Blot.
Reactivity	Guinea Pig, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat Thy 1 antigen. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Specificity	This antibody recognises CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	thymus cell antigen 1, theta
Database Link	Entrez Gene 21838 Mouse UniProt ID P01831
Background	CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.
Synonyms	Thy-1, THY1, CDw90
Reference Data

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