Macrophages / Monocytes Rat Monoclonal Antibody [Clone ID: MOMA-2]

Specifications

Product Data
Clone Name	MOMA-2
Applications	FC, IHC
Recommend Dilution	Flow Cytometry (Membrane permeabilisation is recommended). Immunohistochemistry on Frozen Sections: 0125 μg/ml (1/1600). Recommended Positive Control: Mouse spleen. Does not work on Formalin-Fixed, Paraffin-Embedded Tissue Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse lymph node stroma. Antigen, epitope: The antigen is a (glyco-)protein of 140kDa m.w. which is located within the cytoplasm and on the cell surface. The epitope has not been further characterized.
Specificity	This antibody clone MOMA-2 is a useful marker for the broad detection of monocytes and macrophages in all mouse strains. In combination with the anti F4/80 marker BM8 it allows a precise characterisation of tissue fixed macrophages in various organs. The antibody stains a mature macrophage subset, monocytes and a few precursors in bone marrow. Dendritic cells show low to intermediate expression. The staining shows close correlation with expression of acid phosphatase in tissue sections. MOMA-2 is predominantly expressed in the cytoplasm, but is also present on the cell surface. Antigen Distribution Isolated Cells: In the cytospin preparation of thioglycollate stimulated peritoneal exudate cells MOMA-2 detects an antigen as distinct cytoplasmic spots. MOMA-2 detects monocytes of the peripheral blood and a subpopulation of bone marrow cells. Tissue Sections: MOMA-2 detects typical tissue macrophages as does the anti F4/80-specific clone BM8 (pan macrophage marker). However, different staining patterns are visible as shown below. The most predominant difference can be observed in T-cell areas and follicles of peripheral lymphoid organs where the anti F4/80 clone BM8 is negative.
Isotype	IgG2b
Formulation	Stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon as a preservative Label: Biotin State: Lyophilized purified IgG fraction from cell culture supernatant
Reconstitution Method	Restore by adding 0.5 ml distilled water (= 0.2 mg/ml Stock Solution).
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Synonyms	Macrophage marker, Monocyte marker
Note	This antibody was produced serum-free, without fetal calf serum.
Reference Data

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