CD28 Rat Monoclonal Antibody [Clone ID: YTH913.12]

Specifications

Product Data
Clone Name	YTH913.12
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Specificity	This antibody recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
Isotype	IgG2b
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with distilled water
Purification	Affinity chromatography on Protein G.
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD28 molecule
Database Link	Entrez Gene 940 Human UniProt ID P10747
Background	Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.
Synonyms	TP44
Reference Data

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