CD49b (ITGA2) Mouse Monoclonal Antibody [Clone ID: 16B4]

Specifications

Product Data
Clone Name	16B4
Applications	ELISA, FC, IHC, IP, WB
Recommend Dilution	ELISA: 10 µg/ml. Western Blot. Flow Cytometry: 30 µg/ml; Immunoprecipitation: 25-50 µg/ml. Immunohistochemistry on Frozen Sections: 30-40 µg/ml. Immunohistochemistry on Paraffin Sections (requires antigen retrieval using heat treatment prior to staining of paraffin sections).
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
Specificity	This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
Isotype	IgG1
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 2
Database Link	Entrez Gene 3673 Human UniProt ID P17301
Background	CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.
Synonyms	Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor
Reference Data

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