CD53 Mouse Monoclonal Antibody [Clone ID: MEM-53]

Specifications

Product Data
Clone Name	MEM-53
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leukocytes of pacient suffering from a LGL-type leukemia
Specificity	The antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Isotype	IgG1
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD53 molecule
Database Link	Entrez Gene 963 Human UniProt ID P19397
Background	CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Synonyms	Cell surface glycoprotein CD53, Tetraspanin-25, MOX44, TSPAN25
Reference Data

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