TNFRSF1A (full length) Mouse Monoclonal Antibody [Clone ID: H398]

Specifications

Product Data
Clone Name	H398
Applications	ELISA, FC, FN, IHC, IP, WB
Recommend Dilution	Western blot. Flow Cytometry. Functional assays (antagonistic). Immunoassays. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody H398 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. Does not react with TNF-RII.
Isotype	IgG2a
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	tumor necrosis factor receptor superfamily member 1A
Database Link	Entrez Gene 7132 Human UniProt ID P19438
Background	TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
Synonyms	Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a
Note	Be aware that the antibody competes with TNF-alpha.
Reference Data

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