CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

Specifications

Product Data
Clone Name	Eat2
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	38C13, murine B cell line. Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Specificity	This antibody recognises and CD81. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
Isotype	IgG
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein G.
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD81 antigen
Database Link	Entrez Gene 12520 Mouse UniProt ID P35762
Background	CD81 is a 26kD cell surface glycoprotein that is also known as TAPA-1.In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
Synonyms	S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.