TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

Specifications

Product Data
Clone Name	HTA125
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/25 diluted antibody to label 10e6 cells or 100 µl whole blood. Western blot. Immunoprecipitation.
Reactivity	Canine, Guinea Pig, Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Ba/F3 cell line expressing TLR4. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	toll like receptor 4
Database Link	Entrez Gene 574360 MonkeyEntrez Gene 7099 Human UniProt ID O00206
Background	TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Synonyms	Toll-like receptor 4
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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