CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: F-10-89-4]

Specifications

Product Data
Clone Name	F-10-89-4
Applications	FC, IHC, IP
Recommend Dilution	Flow cytometry: 1/10 - 1/50, use 10 µl of of the suggested working dilution to label 10e6 cells. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/100 - 1/200; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA pH8.0 is recommended for this purpose. Recommended positive control tissue: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human T lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Specificity	This antibody recognises the CD45 cell surface antigen, also known as the leucocyte common antigen. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
Isotype	IgG2a
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 is a complex molecule existing in a number of isoforms.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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