CD146 (MCAM) Rat Monoclonal Antibody [Clone ID: OJ79c]

Specifications

Product Data
Clone Name	OJ79c
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Western Blot: 0.5-1 µg/ml. Immunoprecipitation: 1-2 μg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Positive Control: A-375, HUV-EC or HeLa Cells, Tonsil or Melanoma.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Recombinant Human MUC18 protein.
Specificity	This antibody recognises the 118kD cell surface glycoprotein CD146. Cellular Localization: Cell surface.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	130 kDa
Gene Name	melanoma cell adhesion molecule
Database Link	Entrez Gene 4162 Human UniProt ID P43121
Background	The Human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Synonyms	MCAM, MUC18, CD146
Reference Data

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