Bsg Rat Monoclonal Antibody [Clone ID: OX114]

Specifications

Product Data
Clone Name	OX114
Applications	FC, IHC, WB
Recommend Dilution	Western Blot. Flow Cytometry: Use 10 µl of 1/25-1/50 diluted antibody to label 10e6 cells in 100 µl. This antibody is routinely tested in flow cytometry on Mouse splenocytes. Immunohistochemistry on Frozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Fusion protein mCD147dO-2rCD4d3 +4.
Specificity	This antibody recognises CD147 (also known as basigin). OX-114 recognises both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.
Isotype	IgG1
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	basigin
Database Link	Entrez Gene 12215 Mouse UniProt ID P18572
Background	CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.
Synonyms	Basigin, BSG, EMMPRIN, TCSF
Reference Data

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